​http://bowtie-bio.sourceforge.net/index.shtml

【說明】

【安裝】

   wget http://downloads.sourceforge.net/project/bowtie-bio/bowtie/0.11.3/bowtie-0.11.3-bin-linux-i386.zip?use_mirror=nchc
   unzip -x bowtie-0.11.3-bin-linux-i386.zip

【路徑】

   /opt/bowtie

【測試】

   bowtie -f -m 1 -l 25 -n 2 gl_ref ./bowtie_test_reads.txt output.txt

【結果】

• 輸出檔案：output.txt

  1379_372_66_F3  +       scaffold00001  length=4743      2325    TTCTCAGTCAGTCTTCATGGCAGCA       IIIIIIIIIIIIIIIIIIIIIIIII       0
  1380_574_1082_F3        +       scaffold00001  length=4743      502     TCCTCCACCGCACCTCCTCTCTCTC       IIIIIIIIIIIIIIIIIIIIIIIII       0
  1380_770_1324_F3        -       scaffold00001  length=4743      378     AAGAGGAGAGAGCCGAGAGACGACT       IIIIIIIIIIIIIIIIIIIIIIIII       0
  1380_1073_552_F3        +       scaffold00001  length=4743      843     CACCCAGACTGCGTTCCTTCAACAC       IIIIIIIIIIIIIIIIIIIIIIIII       0
  1380_1389_821_F3        -       scaffold00001  length=4743      4304    TTTCACATGCACACGTCAACACCCA       IIIIIIIIIIIIIIIIIIIIIIIII       0       23:A>T,24:A>T
  1380_1879_1685_F3       +       scaffold00001  length=4743      2081    TCCGAAATATCCTGCGGATCTTATT       IIIIIIIIIIIIIIIIIIIIIIIII       0       2:T>C